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KMID : 0370819930080010079
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Yonsei Journal Dental Science
1993 Volume.8 No. 1 p.79 ~ p.106
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The Effect of Attachment Proteins Coated on Titanium Discs on The Adhesiveness And Proliferation of Human Gingival Fibroblast
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Abstract
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Endosseous dental implants are interfaced with bone, connective tissue, and epithelium when implanted into the jaw bone. The soft tissue interface including connective tissue and epithelium is one of the most critical factors in the determination
of
implant maintenance and prognosis The connecdtive tissue interface not only forms the attachment apparatus, but also contributes to the formation of healthy peri-implant tissue by prevention of the epithelial downgrowth. There are two ways to
promote
connective tissue fibroblasts adhesiveness to implanted biomaterials, one is to control the surface roughness of the biomaterial and the other is to coat the biomaterial with attachment proteins. Between them, coat the biomaterials with
attachment
proteins is denounced. Fibronection and type I collagen are the only attachment proteins studied to titanium biomaterials for evaluation of their effectiveness adn the results were not united. And it is difficult to evaluate the exact effect of
the
attachment proteins due to experimental difficulties and methods of application. So the comparative analytical studies of the effect of attachment proteins under controlled condition are needed.
The purpose of this in vitro study was to evaluate the adhesiveness and proliferation of human gingival fibroblasts to attachment protein-coated and non-coated endosseous titanium biomaterials. Well-known attachment proteins were used, they were
type I
collagen, type IV collagen, fibronectin, laminin, and vitronectin. Each attachment proteins applied onto the commercially pure titanium discs. In this study, the protein-coated and non-coated titanium discs were classified as each groups. Human
gingival
fibroblasts cultured onto each groups. After 30, 60, 180 mins incubation time, unattached cells counted with Coulter counter for evaluation of the adhesiveness of human gingival fibroblasts. The configurations of attached human gingival
fibroblasts
were
done by SEM obsrvation. To confirm the focal contact areas, actin cytochemical and vinculin immunofluorescent staning were done after 3.24 hours of incubation. To evaluate the effect of attachment proteins on the proliferation of human gingival
fibroblasts, 3H-thymidine were added after 72 hours incubation. The results were as follows.
1. Adhesiveness of human gingival fibroblasts were best in fibronectin-coated group among groups (p>0.05). Then laminin, vitronectin, and type IV collagen groups were better, and there were no statistically significant difference between type I
collagen group and control (p<0.05).
2. Fibronectin and type IV collagen groups showed well spread human gingival fibroblasts in SEM observation.
3. Fibronectin group showed most dense and regular arrangement of actin filaments among groups.
4. Fibronectin group showed most distinct vinculin patches along the cytoplasmic process among groups.
5. In the proliferation of human gingival fibroblasts, there no stasistically significant difference among groups (p<0.05).
On the bases of these findings, it its strongly suggest that fibronectin coating at the neck portion of titanium biomaterial promotes adhesiveness of human gingival fibroblasts and suggest the possibility of healthy implant maintenance. Clinical
application of the attachment proteins are further studied and comparative analysis of attachment proteins applied on implant biomaterials against epithelial cells are needed.
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KEYWORD
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